Next Generation Sequencing - Whole Genome Sequencing India

BDG LifeSciences (OPC) Pvt. Ltd. India is a Bioinformatics company and is conducting Training, Novel Research Projects, Workshops & Online Courses in technologies of Bioinformatics since 2010. To date, we have successfully trained hundreds of participants who were not only students but scientists, faculties, professors, company executives, etc from India and other countries. As we believe in a different way of learning which is HANDS-ON PRACTICAL SESSIONS our workshops are unique because participants perform on their own computer/laptop on software/servers, so it is practical application of technology which is the main reason of our success where we have conducted 250+ workshops around the world on latest technologies of Bioinformatics especially Drug Discovery & Designing, MD Simulations, NGS Data Analysis and Gene & Genome Editing by CRISPR/Cas9. To view the pictures of our previous events click here-1 and click here-2 To view the listing of upcoming events kindly click here and our previous workshops can be viewed here. To view the video feedback of our previous events kindly visit our YouTube Channel.

• Introduction
• Experiment
• Sequencing data
• Data QC
• Assembly, QC
• Genome Finishing
• Gene Annotation

PRACTICAL APPLICATION

• Sequencing of unknown genome
• Genome annotation
• Gene function assessment
• Variation assessment in closely related organisms
• Phylogenetic analysis

SCHEDULE

DAY 1

• Introduction
• Workflow
• Data identification
• Data retrieval
• Uploading data to galaxy
• Raw data QC and interpretation.
• Lab workflow: DNA isolation; QC; Library Preparation; QC
• Sequencing data generation
• Data downloading from ENA
• Installation of java on windows and MAC OS
• Installation of FastQC
• Data upload in Galaxy
• Reference-based Alignment using BWA map
• De novo assembly using SPADES.

DAY 2

• Genome finishing using Medusa online server
• Genome annotation using Augustus from Galaxy
• Bacterial genome analysis using Kaiju and CGE online server
• Plasmid analysis using plasmid finder
• Assembly statistics using QUAST using Galaxy and Linux
• Plasmid analysis using different tools
• tRNA analysis using different tools
• Functional annotation using galaxy and different server
• Identification of clinical isolates using online server
• Clustering and functional annotation using cd-hit and cog using online server
• Results analysis from Phaster
• Results analysis from RAST annotation and subsystem classification
• Multiple sequence alignment
• Phylogenetic analysis
• Variant calling and annotation using Galaxy

OUTCOME

• Data analysis on Linux, Windows / MacOS
• Data interpretation
• Various software and visualization tools using R/Python

TARGET AUDIENCE The target audience for this workshop is:
Student & Faculty: Bachelor, Masters, Ph.D., students as well as Faculty and Professors from Microbiology, Biochemistry, Biotechnology, Immunology, Pharmacy, Pharmaceutical Chemistry, Biomedical Technology, Genetics, Bioinformatics, Plant Science and Life Sciences.
Professionals: Biotechnology, Bioinformatics and Pharmaceutical scientists from industry, academia, and regulatory agencies. Hands-on exercises will be performed individually using software tools (no prior experience required).

• Thermal checking will be done at the time of entry for all days of the event.
• Participants should bring their face masks with hand sanitizers.
• Masks are mandatory and participants should wear them all the time.
• Bringing the laptop is mandatory to perform the tasks.
• While buying the ticket choose the right category of registration in which you fall. If you register under the wrong category then your registration will be invalid and NO REFUND WILL BE MADE IN THIS CASE. This is your mistake and the company is not responsible for it.
• Once registration is done it is NON-REFUNDABLE/NON TRANSFERABLE.
• Please bring your PHOTO ID of College/ Department/ Institute/ University/ Company for verification.
• Please bring the PRINT OUT of the ticket which you have got after registration. We need a HARD COPY. Without PHOTO ID and PRINT OUT of ticket entry will not be given.
• Once you register we will send you Welcome email 5 days prior to the event. This email will have all detail of files, software, etc.
• You need to follow the instructions in the welcome email and prepare your system accordingly.
• If you do not turn up for any training session on any day then NO CERTIFICATE WILL BE AWARDED TO YOU.
• Timings are to be strictly followed- If you are late for more than 15 minutes from the start then you will not be allowed to enter on that particular day.
• The certificate will be given only when you attend all sessions and of the category in which you have registered.
• BDG Lifesciences (OPC) Pvt. Ltd., India reserves the right of admission in all our programs.
  If you are removed or your registration is canceled then there will be no answer to that. We have our own reasons for such an act of ours
  If we do not wish to give this workshop to any participant then we will refund their amount.
• Read more points on Terms & Conditions page on the website www.bdglifesciences.com